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32 oz. Bio-Enzymatic Tile and Grout Cleaner

  • Advanced tile cleaner that keeps cleaning long after you’re done
  • Naturally break down trapped dirt & grime using natural enzymes
  • Formula is effective for any surface or finish
  • See More Details

Frequently Bought Together


Product Overview

Dissolve dirt and grime over time with the Rejuvenate Bio-Enzymatic Tile and Grout Cleaner. It uses natural enzymes to make your tile and grout whiter and brighter with every use. The formula continues cleaning long after you are done using it.
  • Dissolves trapped dirt and grime
  • Scrub-free, just spray and walk away
  • For use on any surface or finish
  • Lightens and brightens with every use
  • Naturally breaks down deeply embedded bacteria
  • Keeps on cleaning long after you're done
  • Whitens and brightens grout, removing stains

Info & Guides

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BrandRejuvenateRejuvenateRejuvenateCustom Building Products
Name32 oz. Stone, Tile and Laminate Floor Cleaner32 oz. Bio-Enzymatic Tile and Grout Cleaner16 oz. Floor Restorer and ProtectantTileLab 32 oz. Grout and Tile Cleaner
Recommended SurfacesLaminate,Stone,Stone,TileGrout,Tile,VinylLaminate,Tile,VinylGlass,Grout,Laminate,Tile
Helps RemoveDirt,Dust,Odor,Scuff MarksDirt,DustDirt,Dust,OdorDirt,Food,Grease,Soap (Calcium/ Lime)
FeaturesResidue Free,Streak FreeNo-RinseStreak FreeNon Abrasive
Product FormLiquidLiquidLiquidLiquid
Concentrated or Ready to UseConcentratedReady to useReady to useReady to use
Product Size32 OZ-Ounce32 OZ-Ounce16 OZ-Ounce32 OZ-Ounce
View ProductView ProductView ProductView Product



Product Size
32 OZ-Ounce


Appliance Category
Floor Care
Concentrated or Ready to Use
Ready to use
Dry time (hours)
Regular Strength
Helps Remove
Pack Size
Product Form
Recommended Surfaces

Warranty / Certifications

Certifications and Listings
No Certifications or Listings
Manufacturer Warranty
30-day Money Back Guarantee

Questions & Answers


Safe on grout between marble?

Asked by Steph December 31, 2020

Thank you for the question. Please contact us at RejuvenateProducts.com, by clicking the chat feature, Monday – Friday, from 9:00 am to 5:00 pm (EST) and we would be glad to assist you with any questions you may have about our product.

Would I be able to use a Bissell Steam Mop to occassionally clean the tile and grout?

Asked by RickL October 4, 2020

Honestly - I tried this product to clean very dirty grout and cracks and inlets normal in travertine marble that had gotten dirty. I didn't find this product did any better than either bleach cleaner or simple green (my personal recommendation). We live in a dustbowl and grout and tile is going to get dirty over time. THe steam mop my help with any cleaner on a regular basis to cut down the problem.

Terrazzo tile

Asked by GB August 6, 2020

Yes, this product is safe to use on terrazzo tile. If you have any further questions on comments please feel free to contact us at RejuvenateProducts.com, by clicking the chat feature, Monday – Friday, from 9:00 am to 5:00 pm (EST) and we would be glad to help you.

Can this be used safely on colored grout? I want my grout cleaned, not lightened.

Asked by Tinkbaby January 21, 2020

I love this product. I have colored grout but it is a lighter sand-like shade to begin with (but definitely not white or even close). I haven’t noticed any lightening effect from using it, but I would still suggest testing it in an inconspicuous corner someplace first.

What’s the pH of this cleaner?

Asked by Customer November 18, 2019

Worked good, but I ordered the wrong one. Really needed to order the Rejuvenate Deep Clean.

Can you use with an Electrolux floor cleaner

Asked by Pcf September 11, 2019

Hello, Our Rejuvenate Floor Cleaners are compatible with our Rejuvenate Click n Clean mop system”. Our Rejuvenate Floor Cleaners cannot be used with Electrolux items. I hope this information was helpful. Please contact our customer care division, toll free at (877) 959-1234 so that we may provide you with the assistance you require. Rejuvenate Customer Care Team

How do you use it; with a wet mop, dry mop, do you need to mop the floors prior to using the prod...

Asked by Mac September 4, 2019

Hello - Thank you for your question. It is not necessary to clean your floors before the application. For best results, spray the product generously over the surface and spread evenly with a dry microfiber applicator and allow it to dry completely. Then rinse the surface with a clean damp mop to remove the dirt. We hope this information has been helpful! Rejuvenate Customer Care Team

Is it safe for pets?

Asked by Annie August 31, 2019

I have 3 cats and use it exclusively as my go-to cleaner for those areas of the floor that require extra work. I use it on varigated taupe/light terra cotta color porcelain floor tile throughout the house. You are supposed to use it by spraying it on the messy area and let it dry (like overnight), then reactivated it by utilizing a normal floor clean up method (like with a mop and bucket of warm water and a little floor cleaner.) Please reread directions on label as they may have changed. I sometimes use water and vinegar or dissolve some Borax into the warm water instead. My cats don’t bother with it but you cloud always teat it out first by spraying some on a dinner plate and let it sit there and see if the pets try to lick it. I doubt they would, as I’m sure they can tell by the smell it’s not food. : )

Does this product really work well??

Asked by Rey July 20, 2019

Hello, The grout whitener in our Rejuvenate Bio-Enzymatic Tile and Grout Cleaner makes dirty and dingy grout stains become brighter and lighter after every use. Non-toxic formula is safe for any surface or finish including tile and grout floors, bathroom walls and shower enclosures , no tile scrubber or grout cleaning brush needed. We hope this information has been helpful. Rejuvenate Customer Care Team

Does the cleaner change your grout color? I have pewter grout I want to keep clean but not whiten it

Asked by Diana July 12, 2019

Thank you for your question. Rejuvenate Bio Enzymatic Tile & Grout Deep Cleaner will clean the grout and may lighten the color tone but it should not remove the color. We recommend to test in a small inconspicuous area first and if satisfied with the results then proceed to clean the remaining grout. Rejuvenate Customer Care Team

32 oz. Bio-Enzymatic Tile and Grout Cleaner - page 2

Customer Reviews

  • 3.7
    out of 363 reviews
  • 67% recommend this product
Filter by:
Showing 1-10 of 363 reviews
After receiving Bio-Enzymatic tile and grout cleaner, we finally had a chance to apply it to our ...
After receiving Bio-Enzymatic tile and grout cleaner, we finally had a chance to apply it to our tile flooring and we were genuinely surprised that it worked as described. Easy application as we transferred the liquid to an empty sprayer bottle to evenly distribute as we made sure we had enough to cover all our kitchen tiles and hallway areas that needed some revitalizing. 3 easy steps as we followed the directions. Sprayed small areas, mopped to evenly cover the sprayed tiles and grout, waited for it air dry and then rinsed mop with clean water and then remopped area. Let it dry and tiles and grout showed markedly cleaner than prior to using Bio Enzymatic cleaning solution. We re applied to areas that required more cleaning and we were satisfied with the results. Will definitely purchase and use again as needed.
by ucslaj00
2 people found this helpful
It works pretty good.
My ceramic kitchen floor. Nineteen years old. The grout we found out was never sealed and as life went on, we never sealed it. I tested this tile and grout cleaner on a small intersection of tiles, right in front of the stove. Following directions, the tested area showed in my attached pictures, was able to achieve a visible improvement. So the product works. My concern is that the process requires you to let the product air dry and as you mop later in life, the enzymes continue to work at cleaning. Is this safe for my grand daughter to be crawling on and how about my dogs? What if I want to seal the grout after cleaning? Does the sealer bond or are the enzymes going to eat that as well? The label says it non toxic but ..... In addition, the process for this product is time consuming. It does work though.
by Keith
Pleasant smell and not harsh on hands, No sticky or slippery residue, light sheen on tiles
The Rejuvenate Bio-Enzymatic Title and Grout Cleaner is a non-toxic cleaning solution, with a pleasant smell and not harsh on hands. It can be used with a regular mop or scrubbing mop (i.e Sonic Duo mop); per the instructions, I didn't dilute the solution and just applied it on the tile floor. I was a bit worried because with other brands using full strength leaves the floor either sticky or slippery and the smell is quite over-bearing; with this tile and grout cleaner, the floor wasn't sticky or slippery and the floors have a light sheen to them - almost like the sheen in satin paints. As far as its cleaning ability, i think it performs just as well other tile and grout cleaners; time will tell for the bio-enzymatic ability, which is supposed to lighten the floors with each subsequent use. Either way, I like that it's non-toxic, gets the job done, and my house doesn't have to smell like vinegar ^_^ !
by Jpn80
1 person found this helpful
Works better than expected.
I was looking for an easy to use grout cleaner and I found it with Bio-Enzymatic Tile and Grout Cleaner. I applied the cleaner to the floor with a soft cloth and rub it in good. I then let it sit over night and washed it off. The grout looked better but not by much. I decided to give it another try. I applied the cleaner using a soft scrub brush and worked it into the grout and again, let it sit over night one more time. The next morning after breakfast I washed the floor with plain water to get up all the residue. This time the results were much better as you can see in the photos. I would give it 5 stars if I did not have to do it twice but than again, maybe my floors were dirty since in a high traffic area. I would recommend this product though as it did a good job cleaning.
by jim3000gt
The right product for the job!
I was looking for a floor cleaner that could remove a film that never seems to disappear after many cleanings. The tile floor was installed in 1998, so the white tiles had yellowed over time. I didn't realize how much until I used this product. At first the spraying, spreading, drying, rinsing, drying seem extra work, but it wasn't bad at all. The results seemed ordinary after the first drying, nothing but clean. However,after rinsing and the second dry, I noticed the film was gone and the tiles looked whiter. I'm getting ready to clean the floors again just to see if those enzymes cause more change, but the floors are still CLEAN. I like this for regular use, but I will say I wish the manufacture included a sprayer hose for this large 128 ounce bottle. Pouring it into a sprayer bottle, even using a funnel was difficult and I spilled. That makes the value of buying larger sizes less of a deal and I dislike waste because of bad packaging. Other then that, a really good product.
by Geniebeanie
1 person found this helpful
OK, this product is just GREAT! It does what it says. Just follow the simple instructions on th...
OK, this product is just GREAT! It does what it says. Just follow the simple instructions on the back and 1 - 2 - 3 your grout will become cleaner and look great. Even better, each time you clean your tile floor, the product will activate again and your grout will look better with each cleaning. I clean my floors weekly and use Rejuvenate Tile and Grout Cleaner twice a month. I just can not believe the difference in my grout. - Yes, I would recommend giving this product a try.
by Joe
3 people found this helpful
Worth trying for moderate stains
I bought this hoping to deal with persistent mold on both my kitchen counter tiles and my bathroom shower tiles. The promise of a non-toxic, zero-scrub solution to mold was just too tempting. Turns out it's not quite as good as billed, because this is really a "daily" cleaner, the kind you spray on every day to keep mold at bay. It's not a heavy-duty cleaner, and won't immediately remove really set-in stains. The bottle says you don't need to scrub, so I didn't, and after one application on my shower tiles, absolutely nothing happened. I kept spraying, two or three times a day, and after a few days, I noticed the mold slowly disappearing. Perhaps over many weeks of treatment, the mold will be gone, although by then I'll run out of the product. To put this all another way, if you have seriously dirty tiles, you're going to have to hit them with bleach or another caustic material, put on your rubber gloves, and scrub away as you've always had to.
by Termie
2 people found this helpful
Great Product
Product directions very simple to follow, with subsequent use very good product
by Windrush72
2 people found this helpful
Seems to Really Work!
So I only have tile in my foyer. But there I had several rust spots from a metal planter that had sat there, I used common cleaners, and while they worked somewhat, I had to really scrub to get them as clean as I did (using a green scour pad); but I still had a couple of small spots that I couldn't get out (See 1st Pic--pointing to one of spots, other is to Left & Down from that). This product did the trick (See Pic. 2)! I didn't even let it sit for any time, after spraying! I did use the same green scour pad, but I didn't have to put any pressure at all to get the spots off! The Grout was dirty too with Georgia Clay & the same rust. It seemed to get those cleaner as well, but it's difficult to say, since the grout is so dark to begin with. We have several rentals that we made the mistake of putting tile in, and tile with light grout, no less! We will definitely be using this product on them, on the next turn-over cycle!
by Kristine
3 people found this helpful
Rating provided by a verified purchaser...
Rating provided by a verified purchaser
by HomeDepotCustomer
Showing 1-10 of 363 reviews